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香椿葉抗氧化物提取工藝優(yōu)化與抗氧化活性分析
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    摘要:

    研究了香椿葉抗氧化物（ATSL）的最佳提取工藝及其抗氧化作用,。以總抗氧化能力為指標(biāo),，采用正交試驗(yàn)設(shè)計確定了ATSL的最佳提取條件是：以體積分?jǐn)?shù)50%乙醇為提取溶劑,，料液比0.1g/mL，80℃回流提取2次,，每次3h,。以Fenton反應(yīng)、鄰苯三酚自氧化和DPPH法檢測其清除自由基活性,，結(jié)果表明ATSL對?OH,、O2-?和DPPH?的清除能力呈量效關(guān)系。從ATSL對不同抗氧化反應(yīng)的IC50看,，其對DPPH?的清除能力大于對?OH和O2-?的清除能力,。用硫氰酸銨法評價ATSL 在亞油酸乳狀體系中的抗脂質(zhì)過氧化作用，發(fā)現(xiàn)0.05g/L ATSL抗脂質(zhì)過氧化作用比相同濃度的BHT弱,，比L-抗壞血酸強(qiáng),，與α-生育酚相當(dāng)。

    Abstract:

    The aim of the study was to obtain crude antioxidants from toona sinensis leaves (ATSL) and evaluate its anti-oxidative activity. The optimum extracting condition was investigated by using orthogonal test with total anti-oxidative capacity (TAC) as index, and as follows: 50% ethanol as solvent, ratio of material to liquor was 0.1g/mL, at 80℃ for 3h, and extracted for two times. The anti-oxidative activities, including hydroxyl radicals, super oxide radicals and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were studied. ATSL showed concentration-dependent free radicals scavenging activity. From a comparison of the C50 values for different anti-oxidative reactions, it seemed that ATSL was more effective in scavenging DPPH radicals than scavenging hydroxyl radicals and superoxide radicals. In linoleic acid emulsion, ATSL exhibited less inhibitory effect on lipid peroxidation as assayed by the ammonium thiocyanate method than BHT but stronger than L-ascorbic acid, and matched α-tocopherol at the same concentration of 0.05g/L.

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張京芳,王冬梅,周麗,張強(qiáng).香椿葉抗氧化物提取工藝優(yōu)化與抗氧化活性分析[J].農(nóng)業(yè)機(jī)械學(xué)報,2007,38(8):97-100.[J]. Transactions of the Chinese Society for Agricultural Machinery,2007,38(8):97-100.

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