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乳鏈球菌P-99發(fā)酵乳清生產(chǎn)乳鏈菌肽的研究
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    摘要:

    研究了乳清培養(yǎng)基中乳鏈球菌P-99的細(xì)胞生長(zhǎng)和乳鏈菌肽合成特性,，采用正交試驗(yàn)對(duì)乳清發(fā)酵培養(yǎng)基中的碳源,、氮源和磷源進(jìn)行了優(yōu)化。結(jié)果表明,，乳鏈球菌P-99能夠在沒(méi)有添加物的乳清培養(yǎng)基中生長(zhǎng)并合成乳鏈菌肽,，其細(xì)胞生長(zhǎng)和產(chǎn)物合成可以分別用Monod方程和Luedeking-Piret方程擬合,。培養(yǎng)基優(yōu)化試驗(yàn)結(jié)果表明，在乳清發(fā)酵培養(yǎng)基中添加19g/L乳糖,、0.8g/L甘氨酸,、0.45g/L KH2PO4時(shí)，乳鏈菌肽的合成提高了

    Abstract:

    31.7%,。In this paper, the characteristics of cell growth and nisin production on whey by Streptococcus lactis P-99 were investigated, and concentrations of carbon source, nitrogen source and phosphorus source in whey medium were optimized. The results showed that Streptococcus lactis P-99 could grow well and produce nisin on whey medium without any adjuncts. Based on Monod equation and Luedeking-Piret equation, the kinetic model describing cell growth and nisin production was established. The producing rate of Nisin was improved by 31.7% when 19g/L lactose, 0.8g/L glycine, 0.45g/L KH2PO4 were added to the whey medium.

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羅水忠,潘利華,潘麗軍.乳鏈球菌P-99發(fā)酵乳清生產(chǎn)乳鏈菌肽的研究[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2007,38(11):69-73.[J]. Transactions of the Chinese Society for Agricultural Machinery,2007,38(11):69-73.

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