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不同通氣條件下米根霉發(fā)酵產(chǎn)L-乳酸的代謝調(diào)控
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    摘要:

    研究了不同搖瓶條件對(duì)米根霉發(fā)酵體系中乳酸脫氫酶（LDH）與乙醇脫氫酶（ADH）的酶活變化,，以及乳酸和乙醇轉(zhuǎn)化率的影響,，結(jié)果表明：當(dāng)裝液量從10%增加到30％，乳酸的質(zhì)量濃度及相應(yīng)的LDH活力呈下降趨勢(shì)，而乙醇質(zhì)量濃度及ADH活力隨著裝液量的增加而提高,。搖床轉(zhuǎn)速為220r/min時(shí),，乳酸質(zhì)量濃度最大,，對(duì)應(yīng)的LDH活力也最大,；搖床轉(zhuǎn)速為160 r/min時(shí)，乙醇質(zhì)量濃度與ADH活力較高,。單膜封口發(fā)酵乳酸質(zhì)量濃度與LDH活力分別比雙膜封口發(fā)酵提高64.4％和49.9%,，而雙膜封口發(fā)酵時(shí)，乙醇最大質(zhì)量濃度與最大ADH活力分別比單膜封口發(fā)酵提高

    Abstract:

    30.0%和25.5%,。 The effect of different aeration conditions on fermentation product composition and correlative enzymes activities were investigated, the results indicated that the lactate production and lactate dehydrogenase (LDH) activity were both increased as the medium volumes increased from 10% to 30%, but the alcohol concentration and alcohol dehydrogenase (ADH) activity were both decreased. The maximum production of lactic acid and LDH activity was achieved in cultures shaken at 220 r/min while 160 r/min shaking speed resulted in a higher alcohol concentration and ADH activity. The lactic acid concentration and the activity of LDH were 67.5g/L and 5.83U/mL, respectively, which increased by 64.4% and 49.9% respectively by single film fermentation. The ethanol concentration and the activity of ADH increased by 30.0% and 25.5% respectively, by dual film sealed fermentation compared with single film fermentation.

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鄭志,姜紹通,羅水忠,潘麗軍,李興江.不同通氣條件下米根霉發(fā)酵產(chǎn)L-乳酸的代謝調(diào)控[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2008,39(8):79-83.[J]. Transactions of the Chinese Society for Agricultural Machinery,2008,39(8):79-83.

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