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香菇多糖復(fù)合酶法提取及其脫色工藝優(yōu)
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Technology on Extraction and Decoloration of Lentinan by Composite Enzyme Method
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    研究了復(fù)合酶法提取香菇多糖的最佳工藝條件以及香菇多糖的脫色工藝方法,。采用木瓜蛋白酶,、纖維素酶復(fù)合處理,,通過(guò)正交試驗(yàn)確定了酶法提取香菇多糖的最佳工藝:木瓜蛋白酶與纖維素酶質(zhì)量比為2,酶解反應(yīng)的溫度5℃,,pH值6.5,,反應(yīng)時(shí)間3h,提取率可達(dá)16.1%,;采用正交試驗(yàn)方法確定了最佳脫色工藝條件為:活性炭用量2%,、脫色溫度30℃、酶解液pH值4.0,、脫色時(shí)間90min,,多糖損失率和脫色率分別為

    Abstract:

    8.09%和71.21%。The optimum processing conditions of the lentinan enzymatic extraction and the decoloration method of mushroom polysaccharides were discussed. By using papain and cellulose enzyme complex processing method, the ratio of the volume, temperature, the optimum pH value and role of time for orthogonal experiment were determined respectively. The optimum technology of enzymatic extraction of lentinan was as follows: the quality proportion of papain and cellulose enzyme was 2, the enzyme reaction temperature was 55℃, pH value was 6.5, the reaction time was 3h. The extraction rate reached 16.1 percent under this condition. The optimum condition of decoloration technology by orthogonal experiment was as follows:quantity of activated carbon was 2.0%, temperature of decoloration was 30℃,,pH value was 4.0, the adsorption time was 90min.

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鄒東恢,梁敏,楊勇,王文俠.香菇多糖復(fù)合酶法提取及其脫色工藝優(yōu)[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2009,40(3):135-138. Technology on Extraction and Decoloration of Lentinan by Composite Enzyme Method[J]. Transactions of the Chinese Society for Agricultural Machinery,2009,40(3):135-138.

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