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凍融循環(huán)對甲殼素凝聚態(tài)結(jié)構(gòu)和脫乙酰反應(yīng)的影響
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Effects of Freezing-thawing Cyclic Treatment on Condensed State Structure and N-deacetylation of Chitin
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    甲殼素和質(zhì)量分?jǐn)?shù)為40%的濃NaOH溶液的混合物在-18℃和40℃條件下反復(fù)進(jìn)行凍融處理,,結(jié)果表明慢速冷凍加低溫解凍的凍融循環(huán)處理過程中,冰晶的反復(fù)形成和重結(jié)晶能夠破壞甲殼素凝聚態(tài)特別是晶態(tài)結(jié)構(gòu),。經(jīng)過3次凍融循環(huán)處理,,可使甲殼素在NaOH溶液中完全溶解,獲得甲殼素最終質(zhì)量分?jǐn)?shù)為4%,,NaOH最終質(zhì)量分?jǐn)?shù)約為10%的均相溶液,,為用均相脫乙酰法制備半脫乙酰水溶性甲殼素提供前提。

    Abstract:

    The effects of freezing-thawing cyclic (FTC) treatment on the condensed state structure and N-deacetylation of chitin were investigated. The mixture of chitin and concentrated NaOH solutions (40%) was frozen at -18℃ and thawed at 40℃, repeatedly. Slowly freezing and low temperature thawing cycle process can thoroughly destruct the condensed state structure, especially the crystalline state structure, as a result of the ice crystals’ repeatedly formed and recrystallizated. Furthermore, via three times FTC treatment at -18℃, chitin was completely dissolved in NaOH aqueous solution and the homogeneous solution with a chitin concentration about 4% and a final NaOH concentration of approximately 10% formed, which is likely applied to prepare partial N-deacetylated water-soluble-chitin.

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劉廷國,陳艷,李斌,陳建,黃勁松,盛敏剛.凍融循環(huán)對甲殼素凝聚態(tài)結(jié)構(gòu)和脫乙酰反應(yīng)的影響[J].農(nóng)業(yè)機(jī)械學(xué)報,2009,40(10):140-145.Effects of Freezing-thawing Cyclic Treatment on Condensed State Structure and N-deacetylation of Chitin[J]. Transactions of the Chinese Society for Agricultural Machinery,2009,40(10):140-145.

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