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丁香活性物質提取工藝優(yōu)化與抗氧化活性研究
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江慎華,,講師,博士,主要從事天然產物,、食品營養(yǎng)學和功能性食品研究,,Em ail: [email protected]

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“十一五”國家科技支撐計劃資助項目(2006BAD27B06),、江蘇大學高級專業(yè)人 才科研啟動基金項目(09JDG023),、江蘇大學學生科研項目(07A066,、07A067)和江蘇大學大學 生實踐創(chuàng)新訓練計劃項目(2009072)


Extracting Technology and Antioxidant Activity of  Bioactive Components from Clove
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    摘要:

    對丁香抗氧化活性物質提取工藝及其抗氧化活性進行了研究,。結果表明,, 最佳提取工藝條件為:60%乙醇、提取溫度60℃,、料液比1〖DK〗∶20和提取時間40min,; 在 此工藝條件下抗氧化活性物質提取率為(104804±403)μmol/g,獲得的相應丁香粗 提物具有較強的抗氧化活性,,總還原力弱于相同質量濃度的陽性對照BHT和VC( P<001或P<0001),,FRAP法抗氧化能力和DPPH自由基清除能力弱于相同質量濃 度的VC,強于相同質量濃度的BHT(P<005,、P<001或P<0001),;通過生 物活性追蹤發(fā)現丁香抗氧化活性物質主要存在于其弱極性的乙酸乙酯部分,該部分100μg /mL質量濃度樣液的總還原力達0634±0040,,FRAP法抗氧化能力達0433±0005,,DPP H自由基清除率達(85294±0499)%;相關關系表明丁香抗氧化活性的主要物質基礎為總 多酚和總黃酮,。

    Abstract:

    The extracting technology and antioxidant activity of bioactive components from clove were studied. The results showed that the optimal parameters of the extrac ting technology were as follows, the concentration of ethanol was 60%,,th e extracting temperature was 60℃,the ratio of sample to extracting solution wa s 1〖DK〗∶20 , and the extracting time was 40min. The recovery ratio of bioac t ive compounds from clove was (104804±403)μmol/g in this extracting tec hn ology. The crude extracts obtained from this extracting technology were proved t o have strong antioxidant activity. The reducibility of the crude extracts was w eaker than those of two positive controls (BHT and VC) (P<001 or P<0 001). The FRAP values and the activity of scavenging DPPH radicals of the crude extracts were weaker than those of VC and stronger than those of BHT (P<00 5, P<001 or P<0001). It was found by using the bioassay guided metho d that the bioactive antioxidants of clove were mainly attributed to the ethyl a cetate fraction. The reducibility (OD 700nm) and FRAP values (OD 593nm) of t he sample of the ethyl acetate fraction with the concentration of 100μg/mL we re 0634±0040 and 0433±0005, respectively. The percentage of scavenging DPPH radicals of the same sample was (85294±0499)%. The result of correlat io n analysis showed that the strong antioxidant activity mainly resulted from the high contents of the total phenols and total flavonoids.

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江慎華     ,王書源,馬海樂,王振斌,吳士云,王昌祿.丁香活性物質提取工藝優(yōu)化與抗氧化活性研究[J].農業(yè)機械學報,2010,41(1). Jiang Shenhua yun Wang Changlu,Wang Shuyuan, Ma Haile, Wang Zhenbin, Wu Shi yun, Wang Chang Lu. Extracting Technology and Antioxidant Activity of  Bioactive Components from Clove[J]. Transactions of the Chinese Society for Agricultural Machinery,2010,41(1).

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