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Based on single factor experiment, response surface methodology was used and orthogonal experiment was designed to investigate the effect of extruding parameters on soybean oil yield extracted with enzyme-assisted aqueous extraction processing and cellulose degradability after extrusion and expansion. Statistical analysis system (SAS) was employed to establish regression model and interaction of factors was analyzed. Results showed that the optimal total soybean oil yield is 93.02%±0.29% on response surface with the die length of 20mm, moisture of material of 14.5%, rotate speed for screw of 105r/min, and sleeve temperature of 90℃. Furthermore, There existences a optimal cellulose degradability value of 40.28%±0.43% on response surface when die length is 18 mm, moisture of meal is 15%, rotate speed of screw is 100r/min, and sleeve temperature is 95℃. The degree of cellulose degradability has a significant effect on total oil extraction yield during extrusion and expansion period, total oil extracti on yield during extrusion and expansion was not only dependent on cellulose degradability.