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龍眼多糖超聲波-酶解輔助提取工藝優(yōu)化
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Ultrasonic-enzyme-assisted Extraction Technology of Polysaccharides from Longan Pulp
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    摘要:

    采用Box-Benhnken中心組合試驗設計優(yōu)化龍眼果肉多糖的超聲波-酶解輔助提取工藝,，建立了包括纖維素酶添加量,、超聲波功率、酶解溫度,、pH值和時間的五因素回歸模型,。經(jīng)回歸模型分析并結合驗證試驗,，確定多糖的最佳提取工藝條件為：以龍眼干果肉（含水率8.47%）為原料，選取纖維素酶（酶活大于等于200 U/mg）添加量2000 U/g,、超聲波功率250 W,、酶解溫度55℃、pH值 5.0,、時間60 min,，在該條件下多糖提取率達38.71%，比傳統(tǒng)熱水法、酶法,、超聲波法和微波法分別高9.85%,、6.41%、4.35%和3.99%,，且差異達到顯著水平（

    Abstract:

    P<0.05）,。The effects of the amount of enzyme added, ultrasonic power, enzymolysis temperature, pH value and time on the extraction yield of polysaccharides from longan pulp were optimized by response surface methodology. The optimal technological conditions of extraction by ultrasonic and cellulase were determined, then regression mathematical model was established. The optimal technological parameters are as follows: longan pulp with water ratio is 8.47%, cellulase additive ratios is 2000U/g, ultrasonic power is 250W, enzymolysis temperature is 55 ℃, enzymolysis pH value is 5.0, enzymolysis time is 60min, under such conditions, the polysaccharides extraction rate is 38.71%. This result approximated to the value of mathematical model calculated.

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易陽,張名位,廖森泰,唐小俊,張瑞芬,魏振承.龍眼多糖超聲波-酶解輔助提取工藝優(yōu)化[J].農(nóng)業(yè)機械學報,2010,41(5):131-136. Ultrasonic-enzyme-assisted Extraction Technology of Polysaccharides from Longan Pulp[J]. Transactions of the Chinese Society for Agricultural Machinery,2010,41(5):131-136.

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