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荷葉活性物質(zhì)提取工藝與抗氧化活性研究
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and Antioxidant Activity of Bioactive Components from Lotus Leaf
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    摘要:

    對荷葉活性物質(zhì)提取工藝與抗氧化活性進(jìn)行了研究,。結(jié)果表明,，荷葉抗氧化活性物質(zhì)最佳提取工藝為提取時間50min、提取溫度80℃,、乙醇體積分?jǐn)?shù)60%和料液比1∶20；采用生物活性追蹤法研究發(fā)現(xiàn),，在極性依次增大的正己烷,、乙酸乙酯、正丁醇和水相4個極性萃取組分中,，乙酸乙酯萃取組分抗氧化活性（總還原力,、FRAP法抗氧化能力和DPPH自由基清除能力）最強(qiáng)并具有顯著性差異（P<0.001或P<0.01）；通過驗(yàn)證試驗(yàn)發(fā)現(xiàn)該組分抗氧化能力（OD593和DPPH自由基清除率）均顯著高于陽性對照

    Abstract:

    BHT和GBE（P<0.05）,。 The extracting technology and antioxidant activities of lotus leaf were studied. The results showed that the optimal parameters of the extracting technology were the extracting time was 50min, the extracting temperature was 80℃, the concentration of ethanol was 60% and the ratio of sample to extracting solution was 1∶20. The ethyl acetate fraction possessed the strongest antioxidant activities (P<0.001or P<0.01), i. e., the reducing power, FRAP value and DPPH radicals scavenging capacity among the four increasing polar fractions (hexane, ethyl acetate, butanol and aqueous fractions) through the bio-assay guided method. The OD593 values and DPPH radicals scavenging capacities of this fraction were better than those of the positive controls of BHT and GBE (P<0.05) through the experiments of verification. 

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江慎華,馬海樂,王昌祿,王振斌,李永轉(zhuǎn),廖亮.荷葉活性物質(zhì)提取工藝與抗氧化活性研究[J].農(nóng)業(yè)機(jī)械學(xué)報,2010,41(7):141-146. and Antioxidant Activity of Bioactive Components from Lotus Leaf[J]. Transactions of the Chinese Society for Agricultural Machinery,2010,41(7):141-146.

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