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超聲波預(yù)處理對(duì)燕麥蛋白制備ACE抑制肽的影響?yīng)?/div>
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Oat-seed Protein Pretreatment by Ultrasonic on Preparation of ACE Inhibitory Peptides
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    摘要:

    利用燕麥蛋白制備ACE抑制肽時(shí)先利用超聲波對(duì)燕麥蛋白原料進(jìn)行預(yù)處理研究,。以ACE抑制活性和水解度為指標(biāo)，考察了超聲波功率,、處理時(shí)間,、超聲波工作/間歇時(shí)間對(duì)燕麥蛋白預(yù)處理效果的影響。結(jié)果表明,，超聲波預(yù)處理的最佳工藝參數(shù)為：超聲波功率500W,，處理時(shí)間20min,，超聲波工作時(shí)間2 s,、間歇時(shí)間2 s,，該條件下酶解產(chǎn)物對(duì)ACE的半抑制濃度IC50值從0.533mg/mL降到0.299mg/mL，酶解時(shí)間由常規(guī)酶解的90 min縮短到60 min,；超聲波預(yù)處理參數(shù)變化雖然對(duì)水解度影響不顯著,，但對(duì)ACE抑制活性的影響顯著；驗(yàn)證了超聲波引起的蛋白疏水性增加是導(dǎo)致酶解產(chǎn)物ACE抑制活性顯著改善的重要原因之一,。

    Abstract:

    In order to improve enzymolysis technology for preparation of angiotensin-converting enzyme(ACE) inhibiting peptides from oat-seed protein by alcalase protease, ultrasonic pretreatment of oat-seed protein solution was investigated. Based on the inhibitory activity of ACE and degree of hydrolysis (DH), the effects of ultrasonic power, pretreatment time and the on-off time ratio on ultrasonic pretreatment efficacy were studied. The results show that the optimum conditions of ultrasonic pretreatment are 500W of ultrasonic power, 20min of pretreatment time and 2s/2s of ultrasonic on-off time ratio. Under the optimum conditions, half inhibiting concentration (IC50) value of ACE decreases from 0.533mg/mL to 0.299mg/mL, and the time of enzymolysis reduces from 90min to 60min. Experiments also show that ultrasonic pretreatment parameters have made a significant impact on ACE inhibitory activity while no significant effect on the DH. It is verified that the increase of protein hydrophobicity caused by ultrasound is one of main reasons for significant improvement of hydrolysates ACE inhibitory activity. 

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馬海樂,耿靜靜,駱琳,楊巧絨,朱文學(xué).超聲波預(yù)處理對(duì)燕麥蛋白制備ACE抑制肽的影響?yīng)琜J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2010,41(9):133-137. Oat-seed Protein Pretreatment by Ultrasonic on Preparation of ACE Inhibitory Peptides[J]. Transactions of the Chinese Society for Agricultural Machinery,2010,41(9):133-137.

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