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Purification process of triterpenes in Cyclocarya paliurus by macroporous resins was studied. The suitable macroporous resin was screened, the technological conditions of purification were studied, and the optimum purification processing was obtained by the static adsorption and desorption tests as well as dynamic adsorption and desorption tests. The results show that, in three types of the experimented macroporous resins, D-101 macroporous resin presents the best effect of separation with the adsorption capacity and desorption rate of 57.5mg/g and 96.51%, respectively; through dynamic adsorption experiments, the optimum sampling rate and mass concentration is 2BV/h and 1.5mg/mL respectively; through dynamic desorption experiments, the optimum ethanol content is 50%, with eluting velocity of 2BV/h By macroporous resin, the purity of Cyclocarya paliurus triterpenes is 4.1 times higher than crude extracts, the recovery reaches 74.66%. In conclusion, D-101 resin can be applied to purify triterpenes in Cyclocarya paliurus. 