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The fluorescent is usually used as tracers for spraying deposit analysis in pesticide application study. The recovery rate of the tracers is significant for a reasonable explanation of the spraying performance. Experimental scheme was designed in this work and fluorescent was used as tracers. The scheme comprised of a few spraying factors and different environmental conditions. Rhodamine WT and fluorescein sodium were used for the recovery rate tests and weather condition, shading rate, sampling times and sampler feathers were considered as constraints. The experiments shown, on the condition of the same shading rate, sampling times and samplers, recovery rate of Rohdamine WT was higher than fluorescein sodium. If sampling time was fixed with the same deposition carrier, the increase of shading rate would increase the recovery rate of fluorescent tracer. If shading rate and deposition carriers were kept same, the increase of sampling time would decrease the recovery rate of fluorescent tracers. On the condition of the same shading rate and sampling time, filter papers were more suitable deposition carrier comparing to silver papers.