馬鈴薯渣蛋白抗氧化肽的酶法制備

doi:10.3969/j.issn.1000-1298.[year].[issue].[sequence]

首頁 > 過刊瀏覽>2010年第41卷第Z1期 >198-202. DOI:10.3969/j.issn.1000-1298.[year].[issue].[sequence]

馬鈴薯渣蛋白抗氧化肽的酶法制備
DOI:
                        10.3969/j.issn.1000-1298.[year].Z1.[sequence]
                    
CSTR:
                        
                    
作者:
                        
                        
                    
作者單位:
作者簡介:
通訊作者:
中圖分類號:
基金項目:

of Anti-oxidation Peptide from Potato Pulp

Author:

Affiliation:

Fund Project:

摘要

圖/表

訪問統(tǒng)計

參考文獻

相似文獻

引證文獻

資源附件

文章評論

摘要:

以馬鈴薯渣為原料，采用酶法將薯渣中的蛋白轉(zhuǎn)化為具有抗氧化活性的多肽,。以酶解液對DPPH自由基清除率為酶解效果評價指標(biāo),，從木瓜蛋白酶,、風(fēng)味蛋白酶、中性蛋白酶,、堿性蛋白酶,、胰蛋白酶、胃蛋白酶等6種商業(yè)蛋白酶中篩選出胰蛋白酶為最佳水解用酶,。通過優(yōu)化試驗,，得出薯渣蛋白最佳酶解條件為：底物質(zhì)量濃度4g/（100mL）、加酶量7%,、pH值8.0,、料液溫度50℃、酶解時間90min,。酶解液稀釋20倍后對DPPH自由基的清除率為72%，酶解液清除

Abstract:

DPPH自由基的EC50值為0.155g/（100mL）,。Protease screening was studied to prepare potato antioxidant peptide using the enzymolysis method. With potato dregs as the raw material and the scavenging rate of hydrolysate to the DPPH radical as an index, neutral protease was screened out from the following six kinds of protease: papain, flavor protease, neutral protease, alkaline protease, trypsin and pepsin. The optimum hydrolysis conditions obtained by the experiments were substrate concentration 4 g/(100mL), 7% enzyme-substrate ratio, pH value 8.0, 50°C and 90min reaction time. After being diluted 20 times, the scavenging rate of hydrolysate liquid to the DPPH radical reached 72%. The EC50 value of hydrolysate scavenging the DPPH radical was 0.155 g/(100mL).

參考文獻

相似文獻

引證文獻

引用本文

王蓓,馬海樂,喬瑋.馬鈴薯渣蛋白抗氧化肽的酶法制備[J].農(nóng)業(yè)機械學(xué)報,2010,41(Z1):198-202. of Anti-oxidation Peptide from Potato Pulp[J]. Transactions of the Chinese Society for Agricultural Machinery,2010,41(Z1):198-202.

復(fù)制

文章指標(biāo)

點擊次數(shù):
下載次數(shù):
HTML閱讀次數(shù):
引用次數(shù):

歷史

收稿日期:
最后修改日期:
錄用日期:
在線發(fā)布日期:
出版日期:

期刊瀏覽

EI收錄結(jié)果

引用本文

分享

文章指標(biāo)

歷史

文章二維碼