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雞蛋清卵白蛋白與溶菌酶的兩步超濾法分離
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現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專項資金資助項目（nycytx—41—g22）


Separation of Ovalbumin and Lysozyme from Chicken Egg White Using Two-stage Ultrafiltration Technique
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    摘要:

    研究了兩步超濾法分離雞蛋清中卵白蛋白與溶菌酶的可行性,?；诔瑸V機理分析了超濾過程中初始料液體積分數(shù),、pH值和跨膜壓力等參數(shù)對膜通量,、透過液蛋白質(zhì)質(zhì)量濃度的影響,。在考察膜通量變化確定超濾時間的基礎(chǔ)上，通過超濾試驗獲得最佳工藝條件：初始料液體積分數(shù)6.0%,，pH值2.5,，跨膜壓力0.12MPa。超濾產(chǎn)物純度,、回收率較高,，卵白蛋白、溶菌酶的純度分別為85.72%,、87.21%,，回收率分別為51.36%、62.58%,。試驗結(jié)果表明,，兩步超濾法可用于雞蛋清卵白蛋白與溶菌酶的規(guī)模化,、高效分離,。

    Abstract:

    The possibility of using two-stage ultrafiltration technique to separate ovalbumin and lysozyme from chicken egg white was investigated. Based on the mechanism of ultrafiltration, main factors that affecting the permeate flux and total proteins concentration in permeating liquor, such as initial feed concentration, pH value and transmembrane pressure (TMP) were studied. After ascertaining the ultrafiltration time by examining the change of permeate flux, the optimum ultrafiltration conditions were supposed to be as the follows: initial feed concentration, 6.0%; pH value, 2.5; TMP, 0.12MPa. In these optimum conditions, purity and yield of ovalbumin was 85.72% and 51.36% respectively, and that of lysozyme was 87.21% and 62.58% respectively, implying that separation efficiency was satisfied. The experimental results show that it is feasible for two-stage ultrafiltration technique to separate ovalbumin and lysozyme from chicken egg white in bulk and high efficiently.

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黃群,馬美湖,黃茜,耿放,賀夢麗.雞蛋清卵白蛋白與溶菌酶的兩步超濾法分離[J].農(nóng)業(yè)機械學(xué)報,2012,43(3):146-151. Huang Qun, Ma Meihu, Huang Xi, Geng Fang, He Mengli. Separation of Ovalbumin and Lysozyme from Chicken Egg White Using Two-stage Ultrafiltration Technique[J]. Transactions of the Chinese Society for Agricultural Machinery,2012,43(3):146-151.

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  • 在線發(fā)布日期: 2012-03-17
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