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The plant resources with inhibitory effect on postharvest pathogenic fungi from citrus fruits were screened so as to provide the theoretical basis for the development of botanical fungicide. The experiments investigated inhibitory effects in vitro with 30 kinds of ethanol extracts from Chinese herbs against P. italicum,P. digitatum and Alternaria. Citri by using mycelial growth rate method, and control effects in vivo and storage effects of Valencia orange with Coptis chinensis ethanol extracts. The results showed that 30 kinds of Chinese herbs ethanol extracts have different effects on mycelial growth of three citrus pathogens, and Coptis chinensis ethanol extracts exhibited the best antifungal effect which reached up to 100%. However, no difference was found between Prochloraz treatment and Coptis chinensis treatment （P>0.05). Minimum inhibitory concentration (MIC) of Coptis chinensis ethanol extracts is 0.025g/mL.Control test showed that the control effect of Coptis chinensis ethanol extracts against the mixtures of three kinds of spores achieved 90.87% after 10 d of treatment, and there was no significant difference（P>0.05) between Coptis chinensis treatment and Prochloraz treatment. Preservation test indicated that Coptis chinensis ethanol extracts could obviously decrease the incidence of rotting rate and molding of Valencia orange fruits, increase the good fruit rate, and retard the losses of titratable acidity and vitamin C in fruits. Compared with Prochloraz treatment, there was no difference （P>0.05). These results indicated that in vitro experiments, control effect and preservation test exhibited a good antifungal effect with Coptis chinensis ethanol extracts. Therefore,Coptis chinensis ethanol extracts may have a possibility to replace chemical fungicides and a new pesticide can be developed.