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For automatic determination of acid value and peroxide value of edible oils, using edible oils as raw materials, calibrations were developed and validated for determination of acid value and peroxide value by near-infrared spectrometer with continuous samples injection cell. The correlation coefficient R and the root mean square errors of prediction (RMSEP) of the calibration for acid value were 0.9873 and 0.114mg/g in range of 5500～4600cm-1 with baseline correction point at 6524cm-1 and 4823cm-1 and standard normal variant (SNV). The R and RMSEP for peroxide value of calibration were 0.9958 and 0.90mmol/kg in range of 6050～4450cm-1 with first derivative and Norris filter. The results of blind samples validation were good, the calibrations were workable, throughput of 90 samples can be achieved per hour.