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Quality parameters of fresh meat play an important role in influencing on consumers purchasing power and producers’ economic benefit. Non-destructive, real-time detection system device for assessing fresh pork quality based on VIS/NIR spectroscopy was developed. Working principal and process of the system, hardware composition, and software system were introduced. Hardware system included spectral data collection device, conveyor belt, position detection sensor, control system and computer. Non-destructive, on-line detection software system with compatibility of PC hardware and Windows XP software environment was developed based on Delphi and Matlab language, with the function of spectrum acquired and processed online. The system could collect spectral data automatically, process spectral data, predict the sample quality and display the detection results on real time basis. Finally, the system was used to detect water content of fresh pork in real time basis. The detection results from the experiment yielded satisfactory results. The experimental result shows that this system can be used for non-invasive detection of meat quality in real time basis with high accuracy and reliability as well as repeatability. 