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In order to improve the accuracy, efficiency and automatic degree of existing acquisition techniques of dairy cow’s conformation linear appraisal traits, a research method based on depth image was proposed reconstructed the corresponding part with dairy cow’s conformation linear appraisal traits to measure and acquire linear appraisal indexes. Xtion PRO was chosen as raw data acquisition device. The experiment acquired point cloud from dairy cow’s body random position was completed by data acquisition program compiled based on OpenNI interface, and then measured by using MeshLab. The results showed that it was feasible to use low cost RGBD equipment to acquire a range image of corresponding part with the dairy cow’s conformation linear appraisal traits. And then, according to the point cloud acquired from range image, the dairy cow’s appraisal indexes were reconstructed and acquired. The relative error was less than 9% and the error was less than 10mm.