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Effect of UV-C treatment on rice ageing, especially fatty acid oxidation and antimicrobial activities during storage was investigated. The rice was irradiated at ultraviolet of 4 W/m2 exposure intensity for 0, 10, 30 and 60 min, and then exposed or unexposed. The control rice was 0 min exposure. The changes of activities lipoxygenase-3 (LOX-3), which was the key enzyme of resulting in the rice aging, and malondialdehyde (MDA）of fatty acid oxidation metabolism products, the amount of microorganisms, respiration rate and the permeability of membrane, were researched for illuminating effect of UV-C treatment on rice ageing and obtaining optimal processing parameter. The results showed that UV-C could inhibit LOX-3 activity, and reduce the content of MDA. Among the samples, content of MDA of 30 min irradiating time and unexposed to air was shaved up the most to 62.78%, but the content of MDA of 60 min irradiating time and unexposed to air was slightly increased. In addition, exposed to air immediately could delay effect of UV-C treatment. The content of MDA of exposed to air immediately was steadily reducing and the rate of change was lesser. Similar change rule was found out including the change of the total number of microorganisms, breathing intensity, cell membrane permeability. The result indicated that treatment of 4 W/m2 exposure intensity for 30 min and unexposed to air could effectively inhibit fatty acid oxidation and antimicrobial activities of rice, delay rice aging and improve rice quality and effect of preservation.