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土壤硝態(tài)氮自動檢測平臺研究
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國家自然科學(xué)基金資助項目(31201136,、61134011)和高等學(xué)校博士學(xué)科點專項科研基金資助項目(20110008120023)


Automatic Detection Platform for Soil Nitrate-nitrogen
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    摘要:

    為了快速,、低成本獲取土壤硝態(tài)氮含量,,以自制硝酸根摻雜聚吡咯(PPy(NO-3))離子選擇電極為傳感手段,,自主開發(fā)了多通道土壤硝態(tài)氮自動檢測平臺,。以實驗室配制的質(zhì)量濃度為1~250 mg/L的硝態(tài)氮標(biāo)準(zhǔn)溶液為實驗材料,,分別對平臺響應(yīng)時間,、清洗次數(shù)對平臺標(biāo)定及土壤樣本檢測結(jié)果的影響進(jìn)行了研究,。實驗結(jié)果顯示,,平臺系統(tǒng)用于土壤硝態(tài)氮檢測時的響應(yīng)時間為40 s;進(jìn)行系統(tǒng)標(biāo)定和土壤浸提溶液檢測時,,用下一待測溶液對平臺進(jìn)行清洗2次即可達(dá)到理想效果,,此應(yīng)用方式下得到的平臺檢測結(jié)果與手動方式測得的結(jié)果相當(dāng),且平臺的不同檢測通道之間具有相似的檢測性能和良好的一致性,?;?0個土樣的系統(tǒng)驗證結(jié)果顯示,平臺系統(tǒng)的土壤硝態(tài)氮檢測結(jié)果與紫外分光光度法檢測結(jié)果之間具有較好的相關(guān)性,決定系數(shù)為0.91,。

    Abstract:

    In order to detect soil nitrate nitrogrn content rapidly with low cost, an automatic multi-channel detection platform for soil nitrate nitrogrn was developed. The self-designed PPy(NO-3) ion selective electrode (ISE) was used to sense the nitrate ion in solutions. The platform response time, effects of rinsing time on platform calibration and soil nitrate-nitrogen detection were discussed with a series of standard nitrate solutions, nitrate-nitrogen contents of which were ranging from 1~250 mg/L. Testing results showed that response time of the platform was 40 s when it was used in soil nitrate-nitrogrn detection. During system calibration and soil nitrate extracts determination, rinsing operation for the system could be carried out directly with the next sample solution. And rinsing 2 times could achieve a desirable effectiveness. No significant differences were got between the results detected separately by the platform and manual operation. Also, different channels of the platform possessed similar detection performances and good consistency. The system validation results conducted with 20 soils indicated that satisfied correlation was obtained between the soil nitrate-nitrogen results detected with the platform and ultraviolet spectrophotometric method. The determination coefficient (R2) was 0.91.

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張麗楠,張淼,蒲攀,張集恬.土壤硝態(tài)氮自動檢測平臺研究[J].農(nóng)業(yè)機(jī)械學(xué)報,2013,44(Supp2):220-225. Zhang Li’nan, Zhang Miao, Pu Pan, Zhang Jitian. Automatic Detection Platform for Soil Nitrate-nitrogen[J]. Transactions of the Chinese Society for Agricultural Machinery,2013,44(Supp2):220-225.

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  • 在線發(fā)布日期: 2013-10-21
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