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In order to determine the electric parameters of pulsed electric field(PEF) biological effects, we researched the response of the equivalent circuit model in the PEF based on multi shelled dielectric model of spherical cell. The simulation of the transfer function revealed that the cell outer membrane had low pass filter characteristic and the inner membrane had band-pass filter characteristic. Time domain characteristic of transmembrane potentials on cell outer membrane showed that the pulsed electric field, which had less than 2.83Hz of PEF frequency, more than 69.7kV/m of PEF electric field intensity, and ms/μs class of pulse width, could produce reversible electroporation on cell outer membrane. Extremely low frequency pulsed electric field of 100kV/m, 1Hz and 80ms was used to treat germinating mung beans seedling. It was found that the seed germination process was significantly promoted: on the 4th day of germination, and the shoot length and root length of germinating mung beans seed increased by 65.8% and 80.2% respectively compared with those of the control group.