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蛋白濃度對羅非魚皮明膠高溫干燥成膜的影響
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國家自然科學基金資助項目(31271984)和福建省高校新世紀優(yōu)秀人才支持計劃資助項目(JA11143)


Effect of Protein Concentration on the Film Formation of Tilapia Skin Gelatin at High Temperature
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    摘要:

    為明確魚皮明膠在高溫下的成膜特性,考察了不同蛋白濃度對羅非魚皮明膠可食膜性質的影響,。結果發(fā)現,,在25℃下干燥制備的明膠膜(對照組)其抗拉伸強度和熱收縮率分別高達67.72MPa和 48.75%,膜的固形物溶解率和蛋白溶解率分別為22.97%和15.59%,。然而,,在100℃干燥條件下,當成膜液的起始蛋白質量分數為5%時,,形成的明膠膜其抗拉伸強度和熱收縮率分別降低至8.99MPa和8.13%,,而固形物溶解率和蛋白溶解率分別上升至33.36%和37.47%。起始蛋白質量分數提高至40%時,,上述指標都逐漸接近對照組,。另一方面,根據差示掃描量熱儀和傅里葉變換紅外光譜儀對明膠膜的分析結果,,發(fā)現成膜液中起始蛋白濃度較低時,,蛋白的三股螺旋結構在高溫干燥時容易遭到破壞,結果導致明膠膜機械性能和耐水性能的下降,。

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    With the aim to clarify the film-forming characteristics of fish skin gelatin at high temperature, the effect of protein concentration of film forming solution on the properties of edible films based on the tilapia skin gelatin was investigated. The gelatin was extracted from tilapia skins which were obtained from fresh fish during fillet processing. The obtained gelatin were swollen in distilled water for 30min and then dissolved at 60℃ to obtain the film-forming solutions (FFS). Glycerol as a plasticizer was added at the concentration of 20% of gelatin, and the concentration of gelatin dry basis in FFS was adjusted to 5%, 10%, 20%, 30% and 40%. Air bubbles in FFS were removed by a hybrid mixer. Subsequently, the FFS was cast onto a rimmed silicone resin plate (50mm ×50mm) and dried at 100℃ for 30min, and the obtained films were then conditioned at (25±0.5)℃ and (50±5)% RH for 48h. After having been peeled off, the resulting gelatin films were used for the determination of mechanical properties, water resistance properties and heat shrinkage (HS). Furthermore, the formation mechanism of skin gelatin films was elucidated by electrophoretic analysis, differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FT/IR). As a result, the tensile strength (TS) and HS of gelatin films prepared at 25℃ (Control) could reach up to 67.72MPa and 48.75%, with the matter solubility (MS) and protein solubility (PS) of films was 22.97% and 15.59%, respectively. When film-forming solution with protein concentration of 5% was dried at 100℃, the TS and HS of the resulting films was decreased to 8.99MPa and 8.13%, while the MS and PS was increased to 33.36% and 37.47%, respectively. However, the values above were all gradually close to those of the control with increasing the protein concentration to 40% in the film-forming solution. On the other hand, based on the analyses with DSC and FT/IR for the gelatin films, it was found that the triple helical structure of tilapia skin gelatin was destroyed when the film-forming solution with lower protein concentration was dried at high temperature, resulting in the gelatin films obtained with poor mechanical properties and water resistance ability.

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翁武銀,吳菲菲,大迫一史,蘇文金.蛋白濃度對羅非魚皮明膠高溫干燥成膜的影響[J].農業(yè)機械學報,2014,45(7):209-215. Weng Wuyin, Wu Feifei, Osako Kazufumi, Su Wenjin. Effect of Protein Concentration on the Film Formation of Tilapia Skin Gelatin at High Temperature[J]. Transactions of the Chinese Society for Agricultural Machinery,2014,45(7):209-215.

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  • 收稿日期:2013-08-18
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  • 在線發(fā)布日期: 2014-07-10
  • 出版日期: 2014-07-10
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