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光合細(xì)菌產(chǎn)氫過(guò)程中氮源利用實(shí)驗(yàn)
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國(guó)家自然科學(xué)基金資助項(xiàng)目(50976029),、國(guó)家高技術(shù)研究發(fā)展計(jì)劃(863計(jì)劃)資助項(xiàng)目(2012AA051502)和高等學(xué)校博士學(xué)科點(diǎn)專(zhuān)項(xiàng)科研基金資助項(xiàng)目(20060466001)


Nitrogen Source Utilization of Photosynthetic Bacteria in Process of Hydrogen Production
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    摘要:

    對(duì)從活性污泥中篩選出的光合產(chǎn)氫混合菌群進(jìn)行了光合產(chǎn)氫實(shí)驗(yàn),研究了氮源對(duì)光合細(xì)菌生長(zhǎng)和產(chǎn)氫過(guò)程的影響,,分析了光合產(chǎn)氫過(guò)程中混合菌群對(duì)氮源的利用規(guī)律。結(jié)果表明:光合細(xì)菌生長(zhǎng)過(guò)程中對(duì)氮源有很強(qiáng)的選擇性,無(wú)機(jī)氮源尤其是銨鹽類(lèi)物質(zhì)最易為光合細(xì)菌所利用,,有機(jī)氮源次之,;以(NH4)2SO4為氮源,,添加濃度為7 mmol/L時(shí),菌體生長(zhǎng)最為良好,,在培養(yǎng)24~48 h內(nèi),(NH4)2SO4利用速率最大,,最大消耗速率為0.105 mmol/L,。不同種類(lèi)氮源對(duì)光合產(chǎn)氫混合菌群產(chǎn)氫的影響不很明顯,利用有機(jī)氮源產(chǎn)氫效果好于無(wú)機(jī)氮源,。光合細(xì)菌以(NH4)2SO4為氮源,,添加濃度為3.5 mmol/L時(shí),菌體具有較強(qiáng)的產(chǎn)氫活性,,光合產(chǎn)氫過(guò)程中氮源只在0~48 h內(nèi)有少量消耗,,菌體進(jìn)入產(chǎn)氫高峰期后不再利用氮源。

    Abstract:

    The mixed culture of photosynthetic bacterial was screened from activated sludge to carry out the experiment of hydrogen production. The effect of different nitrogen sources on the growth and hydrogen production of photosynthetic bacteria was studied, and the utilization pattern of nitrogen source in the process of hydrogen production by photosynthetic bacterial was also analysed. The results showed that: the photosynthetic bacteria had a significant selectivity in the process of growth to utilize nitrogen source. Inorganic nitrogen sources, especially ammonium salts was the most vulnerable to be used by photosynthetic bacteria, and organic nitrogen sources followed. Using (NH4)2SO4 as nitrogen source for growth, the best added concentration was obtined, which was 7 mmol/L. The maximum utilization appeared in 24~48 h of cultivated time, and the top consumption rate was 0.105 mmol/L. The impact of different nitrogen type on photosynthetic hydrogen production was not significant and the organic nitrogen was slighely better than inorganic nitrogen. When photosynthetic bacteria utilized (NH4)2SO4 as nitrogen source for hydrogen production and the added concentration was set as 3.5 mmol/L, the photosynthetic bacteria showed better activity of hydrogen production. The nitrogen source was only consumed during 0~48 h in the process of hydrogen production, and the cell no longer utilized the nitrogen source once the cell went into the period of hydrogen-producing peak.

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王毅,周雪花,張志萍,張全國(guó).光合細(xì)菌產(chǎn)氫過(guò)程中氮源利用實(shí)驗(yàn)[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2014,45(10):194-199. Wang Yi, Zhou Xuehua, Zhang Zhiping, Zhang Quanguo. Nitrogen Source Utilization of Photosynthetic Bacteria in Process of Hydrogen Production[J]. Transactions of the Chinese Society for Agricultural Machinery,2014,45(10):194-199.

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  • 收稿日期:2013-10-14
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  • 在線(xiàn)發(fā)布日期: 2014-10-10
  • 出版日期: 2014-10-10
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