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To determine the variation of tillage on carbon dioxide (CO2) and methane (CH4) fluxes from winter wheat fields in Beijing’s suburb, different tillage methods were experimented in Qingyundian Town, Daxing District, Beijing. CO2, CH4 fluxes were measured using static chamber-gas chromatographic techniques during two seasons of winter wheat (October 2011-July 2012 and October 2012-July 2013). The experimental results of the four tillage methods, i.e., no tillage (NT), subsoiling tillage (ST), rotary tillage (RT) and conventional tillage (CT) showed that soils with winter wheat were the emission sources of atmospheric CO2, and the sink of atmospheric CH4 during crop growth seasons. Daily and seasonal variation of CO2 and CH4 fluxes changed significantly for NT, ST, RT and CT treatments, respectively. The maximal daily emission flux of CO2 appeared at 0:00 am, and the minimum emission appeared at 10:00 am. The maximal seasonal emission flux of CO2 appeared at seedling and maturing stages of winter wheat and the minimum emission appeared at winter dormancy stage. Daily and seasonal variation of CH4 fluxes under four tillage treatments showed a significant difference, but no significant variety regulation. The seasonal CO2 flux positively correlated with soil temperature in 0~20cm soil layer, while negative correlation was found between the CO2 emission and soil moisture in 0~30cm soil layer. The seasonal CH4 flux positively correlated with soil temperature in 0~20cm soil layer. In winter wheat fields, the seasonal mean CO2 emission flux demonstrated as CT>RT>ST>NT, and the seasonal mean CH4 absorption flux demonstrated as RT>CT>NT>ST. Compared with RT and CT, NT decreased soil CO2 emission flux by 23.3%~27.1%, and increased soil CH4 absorption flux by over 20%. Generally, all the four treatments (NT, ST, RT and CT) enhanced CH4 assimilation at different levels, and no tillage would be a better tillage practice to reduce CO2 emission for winter wheat fields in Beijing’s suburb.