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A greenhouse muskmelon variety was grown under mulched drip irrigation to investigate the effects of different water and nitrogen levels on nitrogen nutrient absorption, distribution and yield of greenhouse muskmelon, soil NO-3N distribution and accumulation, which can provide theoretical basis for high water-fertilizer use efficiency and the optimal regulation and control of greenhouse muskmelon. Muskmelon plants were subjected to three irrigation levels and three nitrogen levels, and totally were 9 treatments. Treatments were completely arranged according to randomization. Results showed that the above-ground dry matter accumulation and amounts of N accumulation were obtained in the treatment of W2N2 and the content of soil NO-3N was the highest in 0~15cm soil layer after the muskmelon harvest and the content of soil NO-3N decreased with the increase of soil depth. The accumulation of 0~60cm soil NO-3N increased with the increase of nitrogen rate and decreased with the increase of irrigation amount. The yield of muskmelon increased with the increase of irrigation amount and nitrogen rate except for the treatment of W3 and N3. It was concluded that the irrigation level W2 and nitrogen level N2 could be recommended as the best combination of water and nitrogen which can improve muskmelon yield in the production of muskmelon under drip irrigation with plastic film mulched in the experimental area.