0.88),;蒙特卡羅模擬結(jié)果表明,,忽略氣單胞菌在食物接觸表面的存活行為會(huì)高估其在廚房?jī)?nèi)的交叉污染水平。"/>

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氣單胞菌在不同食物接觸表面的存活與交叉污染
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國(guó)家自然科學(xué)基金面上資助項(xiàng)目(31271896),、上海市自然科學(xué)基金面上資助項(xiàng)目(12ZR1420500)和上海市研究生創(chuàng)新基金資助項(xiàng)目(10-14-308-101)


Survival and Cross-contamination of Aeromonas spp. on Different Food Contacts
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    摘要:

    為探討氣單胞菌在不同食物接觸表面的存活狀況,,采用擦拭取樣法測(cè)定氣單胞菌于案板,、刀具表面的恢復(fù)率,并將氣單胞菌分別接種于潔凈和被污染的案板,、刀具表面,,評(píng)定氣單胞菌在不同介質(zhì)表面,、不同時(shí)間下的存活狀況。根據(jù)恢復(fù)率和介質(zhì)表面上氣單胞菌存活實(shí)測(cè)值,,采用@Risk軟件對(duì)介質(zhì)表面實(shí)時(shí)帶菌量的期望值進(jìn)行計(jì)算,,并運(yùn)用一級(jí)線性模型對(duì)氣單胞菌存活特征曲線進(jìn)行擬合。同時(shí),,以案板為交叉污染途徑,,對(duì)氣單胞菌在介質(zhì)表面的存活行為對(duì)交叉污染的影響進(jìn)行了探討。結(jié)果表明:采用擦拭取樣法測(cè)得的氣單胞菌從案板,、刀具表面的恢復(fù)率的最佳分布擬合結(jié)果分別是Normal(0.66,,0.15)和Normal (0.72,0.18),;氣單胞菌在被污染的介質(zhì)表面存活的時(shí)間(7h)長(zhǎng)于其在潔凈介質(zhì)表面的存活時(shí)間(3h或2.5h),,且一級(jí)線性模型可以較好地對(duì)氣單胞菌存活曲線進(jìn)行擬合(R2>0.88);蒙特卡羅模擬結(jié)果表明,,忽略氣單胞菌在食物接觸表面的存活行為會(huì)高估其在廚房?jī)?nèi)的交叉污染水平,。

    Abstract:

    The aim of present work is to study the cross contamination and survival capacity of Aeromonas spp. on different food contacts in the kitchen. The recovery ratios of Aeromonas spp. using swabbing method from cutting boards and knives were firstly examined. Then, the survival of Aeromonas spp. on clean and contaminated cutting boards, stainless steel knives surfaces was studied. Based on the survival data and recovery ratios of Aeromonas spp., expected numbers of Aeromonas spp. on cutting boards and knives were calculated. Mathematical models describing survival of Aeromonas spp. as a function of time were also proposed. Meanwhile, in term of cutting boards as the cross contamination approach, combining survival model and further study were conducted to investigate the influence of the survival of Aeromonas spp. on the cross contamination level. Results indicated that the best fitting of recovery ratios of Aeromonas spp. from cutting boards and knives obtained by @Risk were Normal (0.66, 0.15) and Normal (0.72, 0.18), respectively. And Aeromonas spp. proved to survive longer on the contaminated cutting boards and knives than on the clean ones. Survival data for Aeromonas spp. could be well described by linear model (R2>0.88), and models without survival models included would overestimate the risk associated to bacterial transfer.

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董慶利,王海梅,Pradeep K Malakar,陸冉冉.氣單胞菌在不同食物接觸表面的存活與交叉污染[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2015,46(3):228-234. Dong Qingli, Wang Haimei, Pradeep K Malakar, Lu Ranran. Survival and Cross-contamination of Aeromonas spp. on Different Food Contacts[J]. Transactions of the Chinese Society for Agricultural Machinery,2015,46(3):228-234.

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  • 收稿日期:2014-10-31
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  • 在線發(fā)布日期: 2015-03-10
  • 出版日期: 2015-03-10
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