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食源性致病菌單細(xì)胞觀測(cè)與預(yù)測(cè)的研究進(jìn)展
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國(guó)家自然科學(xué)基金資助項(xiàng)目(31271896、31401608)、上海市科委重點(diǎn)支撐資助項(xiàng)目(13430502400)、上海市科委長(zhǎng)三角科技聯(lián)合攻關(guān)領(lǐng)域項(xiàng)目(15395810900)和國(guó)家食品安全風(fēng)險(xiǎn)評(píng)估中心2015年委托項(xiàng)目


Progress of Observation and Prediction for Foodborne Pathogen Single Cell
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    摘要:

    微生物在單細(xì)胞水平下的生長(zhǎng)普遍具有隨機(jī)性和變異性,,同時(shí)低菌量污染食品所造成的危害風(fēng)險(xiǎn)亦不可忽視,,因此食源性致病菌單細(xì)胞的生長(zhǎng)觀測(cè)及預(yù)測(cè)研究逐漸成為食品預(yù)測(cè)微生物學(xué)及食源性致病菌風(fēng)險(xiǎn)評(píng)估的研究熱點(diǎn),。在分析現(xiàn)有相關(guān)文獻(xiàn)的基礎(chǔ)上,,將目前主要的食源性致病菌單細(xì)胞生長(zhǎng)觀測(cè)研究分為間接推斷和直接觀測(cè)兩類方法,;同時(shí)對(duì)食源性致病菌單細(xì)胞生長(zhǎng)預(yù)測(cè)的模型參數(shù)與建模過程進(jìn)行概述,,比較了傳統(tǒng)預(yù)測(cè)微生物的決定模型與微生物單細(xì)胞的隨機(jī)模型二者之間的區(qū)別,,強(qiáng)調(diào)了單細(xì)胞生長(zhǎng)模型需在隨機(jī)建模的基礎(chǔ)上開展,并通過個(gè)體建模方法進(jìn)一步聯(lián)系其與所在食品環(huán)境之間的關(guān)系,。最后,,分析了食源性致病菌單細(xì)胞生長(zhǎng)觀測(cè)與預(yù)測(cè)的現(xiàn)狀,并展望了其未來與食品安全風(fēng)險(xiǎn)評(píng)估相結(jié)合的發(fā)展趨勢(shì),。

    Abstract:

    The research on the growth of the foodborne pathogen in the single cell level is popular and necessary for its random and variability, which is different from it in the population level. Based on the related research literatures, the observation methods of the pathogen single cell are summarized as indirect and direct methods. The indirect method is based on the turbidity method and theoretical hypothesis, meanwhile,the direct method is using the optical devices to record the division process of the cells. For the character of the growth in single cell level, the single cell growth prediction model should be built on the stochastic model rather than the deterministic model used in the traditional predictive microbiology. Modelling the stochastic division process by the individualbased method makes it possible to explain the relations between the single cell and its living environments. The observation and prediction for foodborne pathogen single cell is the foundation to control the risk of the food with lowcontamination. Analysis indicates that the pathogen single cell growth prediction tends to be combined with food risk assessment.

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董慶利,劉陽泰,蘇 亮,王 忻,劉 箐,丁 甜.食源性致病菌單細(xì)胞觀測(cè)與預(yù)測(cè)的研究進(jìn)展[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2015,46(11):221-229. Dong Qingli, Liu Yangtai, Su Liang, Wang Xin, Liu Qing, Ding Tian. Progress of Observation and Prediction for Foodborne Pathogen Single Cell[J]. Transactions of the Chinese Society for Agricultural Machinery,2015,46(11):221-229.

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  • 收稿日期:2015-10-10
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  • 在線發(fā)布日期: 2015-11-10
  • 出版日期: 2015-11-10
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