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In order to further improve the operation effect and stability of transplanter with chute, the guiding seedling mechanism was chosen as the research object, indoor mixed level uniform design experiment was done under the condition that the length of the connecting rod, initial phase angle of crank and velocity of the 2ZDJ-2 seedling transplanter were made as the experiment factors and lodging rate was made as the experiment indicator. Multivariate non-linear regression model between experiment factors and indicator was built by making statistical analysis of the experiment result, and the influence rule of factorial effect on experiment indicator was analyzed. Optimized solution of experiment factor combination was done within the scope of experiment data by using Matlab software, and repeated verification experiment of optimal factors combination was done. Experiment result showed that the guiding mechanism worked well when the initial phase angle of crank was 70°, the length of the connecting rod was 356 mm, and the velocity was 280 mm/s, under this condition, the erectness percentage was up to 95.5%, and the mechanism worked smoothly.