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丙二醛對牛肉線粒體高鐵肌紅蛋白還原能力的影響
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國家自然科學(xué)基金資助項目（31260380）,、國家現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)（肉牛牦牛）技術(shù)體系資助項目（CARS-38）和甘肅省科技重大專項計劃資助項目（143NKDP020）


Effects of Malondialdehyde on Metmyoglobin Reduction Ability of Bovine Muscle Mitochondria
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    摘要:

    研究了體外孵化條件下脂質(zhì)氧化產(chǎn)物丙二醛對牛肉線粒體膜通透性、微觀結(jié)構(gòu)及其電子傳遞鏈調(diào)控的高鐵肌紅蛋白還原能力和NADH依賴型高鐵肌紅蛋白還原酶活力的影響,。結(jié)果表明：在pH值5.6（模擬貯藏條件）和pH值7.4（模擬生理條件）條件下,，線粒體與丙二醛孵化后膜通透性,、孵化液體熒光強度均比對照組顯著增大（ P <0.05）,，線粒體微觀結(jié)構(gòu)出現(xiàn)腫脹變形,、嵴消失,、空泡狀等現(xiàn)象,。當(dāng)pH值為7.4時,，膜通透性、熒光強度顯著高于前者（ P <0.05）,，說明在生理pH值條件下丙二醛更易與線粒體膜作用進而對其結(jié)構(gòu)造成破壞,。琥珀酸鹽能夠促進牛肉線粒體電子傳遞鏈調(diào)控的高鐵肌紅蛋白還原過程，在pH值5.6和pH值7.4條件下添加琥珀酸鹽孵化9 h后,，高鐵肌紅蛋白相對含量分別減少了21.70%和12.87%（ P <0.05）,。但是在反應(yīng)體系中加入丙二醛孵化9 h后，高鐵肌紅蛋白的還原量分別比未添加時降低了15.68%和3.83%（ P <0.05）,。丙二醛同樣能夠抑制牛肉線粒體NADH依賴型高鐵肌紅蛋白還原酶的活力,，其活力在上述pH值條件下分別降低了48.97%和47.80%（ P <0.05）。綜上可知,，丙二醛能破壞牛肉線粒體結(jié)構(gòu),，抑制線粒體還原高鐵肌紅蛋白的能力,，對新鮮牛肉保持其色澤穩(wěn)定性是不利的。因此,，降低牛肉貯藏過程中脂質(zhì)氧化程度,、維持線粒體正常的呼吸作用，對于保護牛肉色澤穩(wěn)定性具有重要意義,。

    Abstract:

    The effects of malondialdehyde on bovine mitochondrial microstructure and metmyoglobin reduction ability were evaluated by measuring membrane permeability, malondialdehyde binding capacity, microstructure, electron transport chain-mediated metmyoglobin reduction and NADH-dependent metmyoglobin reductase activity. Compared with control groups, mitochondria incubated with malondialdehyde were increased significantly ( P <0.05) from membrane permeability and fluorescence intensity. As a result, mitochondria were swollen, mitochondrial cristae were partially disappeared and some of which were slightly vacuolated. Mitochondria incubated with malondialdehyde at pH value of 7.4 (storage condition) had a greater membrane permeability and higher fluorescence intensity ( P <0.05) than those of at pH value of 5.6 (physiological condition), which indicated that malondialdehyde reacted with mitochondrial membrane more easily under physiological condition, resulting in more serious injury to membrane. Succinate promoted the electron transport chain-mediated metmyoglobin reduction, the relative metmyoglobin contents were decreased by 21.70% and 12.87% ( P <0.05) at pH values of 5.6 and 7.4 compared with control groups (without succinate), respectively. Nevertheless, mitochondrial electron transport chain-mediated metmyoglobin reduction was inhibited when incubated with malondialdehyde, the reduction amounts of metmyoglobin were decreased by 15.68% and 3.83% ( P <0.05) at pH values of 5.6 and 7.4 compared with control groups (without malondialdehyde), respectively. In addition, NADH-dependent metmyoglobin reductase activity was also inhibited by malondialdehyde, which was decreased by 48.97% and 47.80% ( P <0.05) respectively when incubated with malondialdehyde at pH values of 5.6 and 7.4. In conclusion, malondialdehyde could injure the microstructure of mitochondria and inhibit the mitochondria mediated metmyoglobin reduction, which was disadvantageous to color stability of fresh beef. Therefore, it is meaningful to reduce lipid oxidation and maintain mitochondrial respiratory function for protecting color stability during storage.

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陳騁,余群力,韓玲,韓廣星,于春起.丙二醛對牛肉線粒體高鐵肌紅蛋白還原能力的影響[J].農(nóng)業(yè)機械學(xué)報,2015,46(12):253-259. Chen Cheng, Yu Qunli, Han Ling, Han Guangxing, Yu Chunqi. Effects of Malondialdehyde on Metmyoglobin Reduction Ability of Bovine Muscle Mitochondria[J]. Transactions of the Chinese Society for Agricultural Machinery,2015,46(12):253-259.

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  • 收稿日期:2015-05-17
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  • 在線發(fā)布日期: 2015-12-10
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