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Considering the sensitive factor of each parameter differences, in order to analyze the effect of influence parameters of multitemperature refrigerator truck on the cooling process, sensitivity analysis method was used to analyze the cooling influence parameters sensitivity of freezing and refrigerated zone for multitemperature refrigerated trucks. The result shows that: for refrigerated zone, when δi is 100mm, λi is 0.0338W/(m·K) to 0.0438W/(m·K), the weights shows an inverse trend which decreases from 0.564 to 0.477; when δi is 20~200mm, the corresponding weight is 0.448~0.126; when Ff is 0.01~0.02m2, the corresponding weights is 0.28~0.113, the heavy weights of other parameters are negligibly small; when δg,vw, Q0, vf,Ff,λi, δi, β1 and H increased by 30%, the corresponding changing rates of weight are -32%,-36%,-30%,-37%,-34%,-17%,-30%,1% and 0.5% respectively. Whereas, when δg, vw, Q0 , vf, Ff, λi, δi, β1 and H decreased by 30%, the corresponding weight changing rates are 90%, 58%, 42%, 78%, 56%, 17%, 44%, -1% and 0.5% respectively. The weight changing rates of the freezing and refrigerated zone are almost the same. When vw, Q0, vf, Ff, δg, λi, δi and β2 increased by 30%, the corresponding weight change rates of all the sensitive factors gradually increases in the negative direction except β2, which increases in the positive direction, the corresponding weight changing rates are -23%,-39%,-35%,-29%,-46%,-19%,-20%,-5%. When vw, Q0, vf, Ff,δg, λi, δi and β2 decreased by 30%, the corresponding weight change rates of all the sensitive factors gradually increases in the positive direction except β2, which increases in the negative direction, the corresponding weight changing rates are 104%, 50%, 66%, 54%, 138%, 20%, 25%, 5%, respectively.