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To study the effects of long-term conservation tillage (no-tillage and subsoiling) on nitrogen accumulation and translocation characteristics of winter wheat, a ten year (2006—2016) field experiment was carried out, and the effects of different tillage treatments, including traditional tillage, no-tillage and subsoiling on the soil water content in 0~100cm during the key winter wheat growth stages were analysed. The results showed that long-term no-tillage and subsoiling treatments significantly increased the soil water content in 0~100cm and no-tillage treatment was superior to subsoiling treatment in the dry year. The nitrogen distribution ratio in stem and leaf was gradually decreased, while it was gradually increased in wheat are from flowering to harvesting stage under three tillage treatments. Compared with conventional tillage treatment, average nitrogen accumulation amounts in stem, leaf and ear under 2-year continuous notillage treatment were increased by 44.3%, 80.5% and 70.9%, respectively, and there was no significant increase under 2-year continuous subsoiling treatment at flowering stage. At harvesting stage, no-tillage and subsoiling treatments significantly decreased the nitrogen accumulation in stem, while significantly increased its ear accumulation amount compared with conventional tillage treatment. Moreover, notillage treatment significantly increased the nitrogen translocation amount of winter wheat vegetative organs, translocation ratio, contribution ratio to grain (P<0.05), while subsoiling treatment was non-sustainable to increase nitrogen translocation amount and ratio. In general, long-term effect of no-tillage treatment on nitrogen translocation of winter wheat was superior to that of subsoiling treatment, especially in the dry year.