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The reserve resources for cultivated land should be targeted to differentiated protection and development management on the basis of different resource conditions and utilization direction, so as to realize the best benefit of capital investment, and then achieve the ultimate goal of coordinated development in research area. Heilongjiang Province was the main grain production area and the most important agriculture province in China. Its grain production and agriculture had obvious advantages. Heilongjiang Province owned large area of reserve resources for cultivated land, which was the most important province in farmland supplement. Taking Heilongjiang Province as the research area, the conditions of resource reserve, resource investment and resource development potential were considered. The spatial analysis and twodimensional graph theory clustering method were used to study the land use zoning and determine the direction of regulation. The results showed that the twodimensional graph theory clustering method can achieve the goal of ensuring the independence of the reserve land use resources and the internal consistency of the zoning. Heilongjiang Province was divided into potential development zone, ecological environment construction zone, key development zone, ecological environment protection zone, development and utilization of control zone and the appropriate development zone, all the six partitions results were serving for the Heilongjiang Province reserve resources for cultivated land. And then different development management strategies were adopted for different zone types, and management strategy was formulated. The research can provide decision support for the development and utilization of reserve resources for cultivated land in Heilongjiang Province.