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Aiming to investigate the effects of DHPM treatment on allergenicity, digestibility and structures of EWP, EWP was processed under a continuous pressure array of 40MPa, 80MPa, 120MPa, 160MPa and 200MPa. After DHPM treatment, the degree of hydrolysis and digestibility of EWP was evaluated by in vitro digestion test, the allergenicity was measured by ELISA assay, and changes in molecular weight and conformational structure of EWP were characterized by SDS-PAGE, circular dichroism spectrum and fluorescence spectra. The results showed that DHPM treatment with pressure ranging from 80MPa to 200MPa could significantly reduce the allergenicity of EWP, especially at 120MPa.The Ig E-binding of DHPM treated EWP and its hydrolysates were reduced by 63.7% and 93.5%, respectively. Meanwhile, the molecular weight distributions of DHPM treated EWP were not changed. However, the DHPM treatment contributed to an increase in relative fluorescence intensity, but the spectral peaks shift was not observed. At the same time, the surface hydrophobicity and free sulfydryl group content of EWP were significantly increased after DHPM treatment (80~200MPa) compared with native EWP, and some α-helices were destroyed and converted to β-sheets and random coils, indicating that the secondary and tertiary structures of EWP were changed. It was found that the reduction of antigenicity was correlated with the changes in the structure and epitopes of the allergenic protein. Thus the content of α-helices was decreased with the reduction in antigenicity, suggesting that Ig E-binding epitopes might be located in the α-helices structure of EWP. Moreover, hydrolysis efficiency and digestibility in vitro of EWP were markedly increased through the DHPM treatment (80~200MPa). These results suggested that DHPM can be adopted as an important physical modification method for EWP of hypoallergenic and improving digestibility. The research can provide theoretical basis for the development of low sensitivity egg white products and their applications in food industry.