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基于臥式厭氧裝置的稻秸高固態(tài)消化與甲烷菌變化研究
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國家自然科學(xué)基金項目(51678279、21506076)、“十二五”國家科技支撐計劃項目(2014BAC25B01)和常州工程職業(yè)技術(shù)學(xué)院校級科研基金項目(11130100116022)


High Solid-state Digestion of Rice Straw and Changes of Methanogens in Horizontal Anaerobic Reactor
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    摘要:

    針對單一稻秸高固態(tài)厭氧消化長期運行不穩(wěn)的問題,接種瘤胃內(nèi)含物和厭氧污泥,在臥式反應(yīng)裝置中研究3個有機(jī)負(fù)荷(OLR)下消化特性,。結(jié)果表明,體系最高容積產(chǎn)氣率達(dá)到了1.04L/(L·d)。當(dāng)OLR為2.26g/(L·d)時,,甲烷體積分?jǐn)?shù)均值為5439%,甲烷產(chǎn)率為280.90mL/g,,達(dá)到了稻秸理論產(chǎn)值的80.29%,。臥式裝置中半纖維素和纖維素最高降解率分別達(dá)到了49.71%和31.25%;纖維素酶活性顯著提高,,有利于纖維素的降解,。當(dāng)OLR升高到2.47g/(L·d)時,氨氮質(zhì)量濃度均值達(dá)到了1082.63mg/L,。固體樣品中嗜氫型Methanobacteriales數(shù)量從1.70×109拷貝數(shù)/g下降至1.04×106拷貝數(shù)/g,;而嗜乙酸型Methanosarcinales數(shù)量從7.89×106拷貝數(shù)/g增加至9.44×106拷貝數(shù)/g,,甲烷產(chǎn)率下降為256.54mL/g。此時厭氧裝置中丙酸質(zhì)量濃度均值達(dá)到了253.32mg/L,。從而明確了稻秸高固態(tài)體系中產(chǎn)甲烷菌結(jié)構(gòu)的變化,。

    Abstract:

    Instability of high solid-state anaerobic digestion often happened during the longterm operation of reactor. To investigate underlying methanism of this problem, the characteristics of semicontinuous anaerobic digestion for rice straw were studied with three organic loading rates (OLR) in a horizontal reactor, when being co-inoculated with ruminal contents and anaerobic sludge. The results indicated that the highest volume biogas production reached 1.04L/(L·d) in the reactor. The average CH4 productivity reached 280.90mL/g VS with 54.39% CH4 volume content at the OLR of 2.26g/(L·d), which was up to 80.29% of the theoretical value of rice straw. The highest digestion rates of hemicellulose and cellulose were 49.71% and 31.25%, respectively. And the cellulase activities were increased obviously in the horizontal reactor, which was indispensable to the degradation of cellulose. At the OLR of 2.47g/(L·d), the average concentration of ammonium nitrogen reached 1082.63mg/L. Especially the highest concentration reached 1196.37mg/L after 192d of operation. It was indicated that the methanogens suffered different influences from ammonium nitrogen using quantitative realtime PCR(Q-PCR). Specifically, the hydrogenotrophic Methanobacteriales was decreased to 1.04×106 copies/g from 1.70×109copies/g dominated in the solid residues and CH4 productivity was decreased to 256.54mL/g for being inhibited significantly. In contrast, the acetotrophic Methanosarcinales was increased to 9.44×106 copies/g from 7.89×106 copies/g. At the same time, the average propionate concentration reached 253.32mg/L in the anaerobic reactor. Thus the study revealed the adaptive shifts of underlying methanogenic communities by ammonium nitrogen inhibition during the high solidstate digestion of rice straw.

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鄧玉營,阮文權(quán),黃振興,繆恒峰,趙明星,施萬勝.基于臥式厭氧裝置的稻秸高固態(tài)消化與甲烷菌變化研究[J].農(nóng)業(yè)機(jī)械學(xué)報,2017,48(7):272-279. DENG Yuying, RUAN Wenquan, HUANG Zhenxing, MIAO Hengfeng, ZHAO Mingxing, SHI Wansheng. High Solid-state Digestion of Rice Straw and Changes of Methanogens in Horizontal Anaerobic Reactor[J]. Transactions of the Chinese Society for Agricultural Machinery,2017,48(7):272-279.

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  • 收稿日期:2017-05-16
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  • 在線發(fā)布日期: 2017-07-10
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