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After 3d or 5d storage at temperature of (3±0.5)℃ under high oxygen atmosphere, Agaricus bisporuswas packaged (modified atmosphere packaging) and placed at (3±0.5)℃ (simulated supermarket fresh cabinet) and (20±0.5)℃ (simulated room temperature sales). The objects were to research the subsequent effects of high oxygen atmosphere on the shelfquality of Agaricus bisporusby measuring the whiteness, hardness, weight loss rate, respiration rate and soluble sugar content. Results showed that Agaricus bisporus after treatment in high oxygen atmosphere could still maintain good quality at (3±0.5)℃. The effect was better than that stored under high oxygen environment all the time. The quality of Agaricus bisporus stored after 3 d was better than that after 5 d. The best quality appeared after treatment in 80% O2.The quality of that refrigerated for 5d at (20±0.5)℃ was the best, which was followed by those of 100% O25d, 100%O23d and refrigerated for 3d. The quality deterioration of Agaricus bisporuswas accelerated under 80%O2 treatment at (20±0.5)℃. Therefore, the results indicated that the subsequent effects on the shelfquality of Agaricus bisporus stored in 80% O2 had preferable influence, while the quality deterioration at (20±0.5)℃ was obvious. When placed at (3±0.5)℃ and (20±05)℃, the packaged Agaricus bisporusafter treatment in 80% O2 were obviously different in whiteness. By comparison, the packaged Agaricus bisporus after treatment in 100% O2and refrigerated was more suitable to distribution at ambient temperature. Thereby, the research provided certain theoretical basis for the preservation and sale of Agaricus bisporus.