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青羊參凝乳劑提取及其對乳餅制作工藝的影響
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云南省科技計劃生物重大專項(奶業(yè)專項)(2014ZA001)


Extraction of Cynanchum otophyllum Schneid. Coagulant and Its Effect on Milk Cake Manufacture
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    摘要:

    以青羊參葉為原料,研究了其有效凝乳成分的提取方法,,并在乳餅加工過程中進(jìn)行了驗證。結(jié)果表明,,青羊參凝乳劑的最佳提取溫度為4℃,最佳提取的液料比為20mL/g,,最佳提取溶液為10mmol/L檸檬酸-磷酸緩沖液,;與傳統(tǒng)直接添加青羊參葉浸泡液的方法相比,使用青羊參凝乳劑制成的乳餅蛋白質(zhì)回收率,、脂肪回收率和乳餅得率均顯著提升(P<0.05);質(zhì)構(gòu)和微觀結(jié)構(gòu)顯示,,使用青羊參凝乳劑制作的乳餅具有更適宜的硬度和較高的粘著度,,凝膠網(wǎng)絡(luò)結(jié)構(gòu)分布均勻。

    Abstract:

    Cynanchum otophyllum Schneid. contains the coagulant protease. The leaves of Cynanchum otophyllum are widely used to produce the milk cake (a traditional cheese) by the minority ethnic region in Yunnan Province. Milk cake made by Cynanchum otophyllum leaves usually forms a soft curd. The object was to extract the active coagulant components based on the leaves of Cynanchum otophyllum and apply the extract into milk cake manufacture. The results showed that the optimal extraction temperature to produce Cynanchum otophyllum coagulant was 4℃, while the optimal extraction liquid-solid ratio was 20mL/g and the optimal extraction solution was the citric acid-phosphate buffer (containing 150mmol/L NaCl, 1mmol/L Cys and EDTA). Compared with the milk cake made by the soaking solution of Cynanchum otophyllum leaves, the yield, protein and fat recovery rate of milk cake made by the extracted coagulant were significantly improved, with the recovery rate for protein and fat of 20.26 and 33.04 percentage points, respectively. Moreover, the milk cake made by extracted coagulant showed a more suitable hardness, higher degree of adhesion and more uniform gel network structure compared with the milk cake made by soaking solution. The study provided a reference for the development of Cynanchum otophyllum coagulant and promoted the industrialization of Yunnan traditional milk cake products.

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穆碩,羅潔,楊子彪,肖晨,桑躍.青羊參凝乳劑提取及其對乳餅制作工藝的影響[J].農(nóng)業(yè)機(jī)械學(xué)報,2018,49(3):367-372. MU Shuo, LUO Jie, YANG Zibiao, XIAO Chen, SANG Yue. Extraction of Cynanchum otophyllum Schneid. Coagulant and Its Effect on Milk Cake Manufacture[J]. Transactions of the Chinese Society for Agricultural Machinery,2018,49(3):367-372.

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  • 收稿日期:2017-08-22
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  • 在線發(fā)布日期: 2018-03-10
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