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Land use of plain-hill-wetland blend area in Sanjiang Plain was changed significantly, resulting in the decline of groundwater level and shortage of water resources, while rare research was focused on revealing the effect of land use change on water resources, especially on the temporal and spatial distribution of water resources of surface water and ground water, wetland water and green water. A plain-hill-wetland blend area hydrological model was constructed based on distributed TOPMODEL and wetland flow kinematic model. The land use change and its impact on water resources in Naolihe River Basin was analyzed by using GIS and RS technology, combined with the geo information and spatial autocorrelation method. The results showed that the areas of grassland and woodland in the Naolihe River basin were changed little, while dry land area was decreased slightly according to the change of cultivated land area and dynamic degree distribution between 1990 and 2013;unused land and dry land with advantageous conditions for production was converted to paddy field in the central and northern areas of Naolihe River Basin;The influence of land use change on water resources declined in the order of withdraw water quantity, green water reserves, runoff flow, green water flow, and groundwater recharge;the evaporation capacity of paddy field was different in different rice growth periods, which led to the increase of green water flow, decrease of green water reserves, decrease of runoff flow, increase of groundwater recharge and increase of withdraw water quantity during May and June in Naolihe River Basin. The difference of these items was decreased gradually during July and August, and disappeared after August. Green water flow was increased, green water reserves and runoff flow were decreased, while groundwater recharge and withdraw water quantity were increased in paddy field.