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便攜式牛乳蛋白質(zhì)含量檢測儀設(shè)計與驗證
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國家自然科學(xué)基金面上項目(31671935)


Design and Validation on Portable Detector for Protein Content in Milk
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    摘要:

    為了給牛乳蛋白質(zhì)含量檢測提供一種操作簡便,、成本低廉的便攜式儀器,基于595nm下考馬斯亮藍(lán)G-250染料中的疏水基團(tuán)在酸性條件下與蛋白質(zhì)的疏水微區(qū)具有較強(qiáng)親和力,,從而影響光透射特性的原理,,設(shè)計了一種便攜式牛乳蛋白質(zhì)含量檢測儀。該檢測儀的硬件主要由單片機(jī),、光源模塊,、光傳感器模塊、輸入輸出模塊和電源模塊等組成,;軟件由Keil C51 編寫,,并由主函數(shù)、光照度采集子函數(shù),、數(shù)據(jù)預(yù)處理子函數(shù),、顯示子函數(shù)和蛋白質(zhì)含量計算子函數(shù)等組成。以生鮮牛乳為對象,,研究了透射光照度與蛋白質(zhì)含量之間的關(guān)系,,發(fā)現(xiàn)隨著蛋白質(zhì)含量的增加,透射光照度減小,,二者具有良好的線性關(guān)系,,其線性決定系數(shù)為0.872。對比該儀器的蛋白質(zhì)檢測結(jié)果表明,,同凱氏定氮法相比,,本檢測儀的絕對誤差范圍是±0.08g/(100g),平均絕對誤差為0.05g/(100g),。此外,,該檢測儀的檢測時間小于2s,可快速,、有效地檢測牛乳中蛋白質(zhì)含量,。

    Abstract:

    Protein content is one of the most important quality indicators of milk. The traditional methods used to measure protein content in milk have high precision, but the measurement procedures are tedious and timeconsuming, and the measurement equipments are expensive. In addition, these methods can not be used in field or insitu measurement. Therefore, developing a kind of portable milk protein content detector with simple operation and low cost, which could be used insitu measurement, would be appreciated by milk producers, processers and consumers. Since the hydrophobic grouping of Coomassie brilliant blue G-250 has strong affinity for protein in acids, a portable milk protein content detector was developed based on light transmission. The hardware of the detector consisted of the modules of single chip microcomputer, light source, light sensor, input/ouput and power. The software was developed by C51 language, and was made up of main function and several subfunctions to realize light collection, data preprocessing, protein content calculation and display. Raw fresh milk was used to study the relationship between intensity of transmitted light and protein content. It was found that the intensity of transmitted light was decreased with the increase of protein content, and they had strong linear relationship with the coefficient of determination of 0.872. The validation experiment on the developed detector showed that contrasted with Kjeldahl method, the absolute measurement error of the developed detector was within the range of ±0.08g/(100g), and its mean absolute error was 0.05g/(100g). In addition, the measurement result could be given in 2s.

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楊彪,王凱,韓天宇,朱新華,郭文川.便攜式牛乳蛋白質(zhì)含量檢測儀設(shè)計與驗證[J].農(nóng)業(yè)機(jī)械學(xué)報,2019,50(3):317-322. YANG Biao, WANG Kai, HAN Tianyu, ZHU Xinhua, GUO Wenchuan. Design and Validation on Portable Detector for Protein Content in Milk[J]. Transactions of the Chinese Society for Agricultural Machinery,2019,50(3):317-322.

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  • 收稿日期:2018-09-28
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  • 在線發(fā)布日期: 2019-03-10
  • 出版日期: 2019-03-10
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