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超高壓下大豆親脂蛋白-羥丙基甲基纖維素理化性質(zhì)研究
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中國博士后科學(xué)基金面上項目（2018M631902）和國家自然科學(xué)基金重點項目（31430067）


Physicochemical Properties of Soy Lipophilic Protein-Hydroxypropyl Methylcellulose Complex under Ultra-high Pressure
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    摘要:

    大豆親脂蛋白（Lipophilic protein, LP）和羥丙基甲基纖維素（Hydroxypropyl methylcellulose, HPMC）在中性條件下（pH值74）復(fù)合后,，可自發(fā)組成LP-HPMC復(fù)合物,，但仍有部分未自組裝的LP和HPMC存在于溶液中，目前仍無法實現(xiàn)LP-HPMC最大程度復(fù)合以及最多結(jié)合位點的目標(biāo),。采用熒光光譜和紅外光譜研究了不同超高壓處理對LP-HPMC復(fù)合程度以及作用力的影響,，采用乳化活性和乳化穩(wěn)定性測定,、表面疏水性分析、動態(tài)光散射粒度分析等解析了超高壓對LP-HPMC結(jié)構(gòu)變化與功能性質(zhì)表達的構(gòu)效關(guān)系,。結(jié)果表明：LP-HPMC復(fù)合物通過非共價相互作用結(jié)合,，蛋白二級結(jié)構(gòu)發(fā)生相應(yīng)改變。當(dāng)壓力為400MPa時,，復(fù)合物有最大的溶解度和最小的表面疏水值,，分別為41.1%和57.832，此時乳化活性和乳化穩(wěn)定性指標(biāo)也最佳。

    Abstract:

    Soy lipophilic protein (LP) and hydroxypropyl methylcellulose (HPMC) can form an LP-HPMC complex spontaneously after being combined under neutral conditions (pH value 7.4), but there are still some selfassembled LP and HPMC presented in solution. At present, the research still can not achieve the maximum compounding degree of LP-HPMC and the target of the most binding sites. Fluorescence and infrared spectroscopy were used to study the effects of different ultrahigh pressure treatments on the degree of LP-HPMC compounding and the force. The effects of ultrahigh pressure on structureactivity relationship of structural changes and functional properties of LP-HPMC were analyzed by emulsion activity and emulsion stability measurement, surface hydrophobicity analysis and dynamic light scattering particle size analysis. The results showed that the LP-HPMC complex was bound by noncovalent interaction and the secondary structure of the protein was changed accordingly. When the pressure was 400MPa, the maximum solubility and minimum surface hydrophobic value of the composite were 41.1% and 57.832, respectively, and the emulsification activity and emulsion stability index were also the best.

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李楊,鐘明明,廖一,謝鳳英,孫禹凡,齊寶坤.超高壓下大豆親脂蛋白-羥丙基甲基纖維素理化性質(zhì)研究[J].農(nóng)業(yè)機械學(xué)報,2019,50(12):365-371. LI Yang, ZHONG Mingming, LIAO Yi, XIE Fengying, SUN Yufan, QI Baokun. Physicochemical Properties of Soy Lipophilic Protein-Hydroxypropyl Methylcellulose Complex under Ultra-high Pressure[J]. Transactions of the Chinese Society for Agricultural Machinery,2019,50(12):365-371.

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  • 收稿日期:2019-04-07
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  • 在線發(fā)布日期: 2019-12-10
  • 出版日期: 2019-12-10
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