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大豆-乳清混合蛋白對(duì)O/W乳液穩(wěn)定性及流變性的影響
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黑龍江省自然科學(xué)基金項(xiàng)目(LH2019C032)和國(guó)家自然科學(xué)基金項(xiàng)目(31801579)


Effect of Soybean-Whey Mixed Protein on Stability and Rheological Properties of O/W Emulsion
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    摘要:

    采用大豆分離蛋白-乳清分離蛋白(SPI-WPI)作為乳化劑制備O/W(水包油)乳液,通過測(cè)定粒徑,、Zeta電位,、乳化活性指數(shù),、乳化穩(wěn)定性系數(shù),、乳液穩(wěn)定性系數(shù),、掃描電鏡,、流變等指標(biāo),,探究不同蛋白混合比例及濃度對(duì)復(fù)合乳液穩(wěn)定性及流變特性的影響,。結(jié)果表明:當(dāng)SPI-WPI乳液蛋白質(zhì)量分?jǐn)?shù)為2.0%,、SPI與WPI質(zhì)量比為1∶9時(shí),乳液體積平均粒徑最小,,為288.56nm,,Zeta電位絕對(duì)值達(dá)到最大,為35.0mV,,乳化活性指數(shù)最大,,為108.23m2/g,乳化穩(wěn)定性指數(shù)最大,,為3.78471min,,穩(wěn)定性系數(shù)最大,為93.59%,,此時(shí)乳液穩(wěn)定性最好,。當(dāng)SPI-WPI乳液蛋白質(zhì)量分?jǐn)?shù)為2.0%、SPI與WPI質(zhì)量比為9∶1時(shí),,乳液的粘度最大,,乳液的剪切應(yīng)力最大,流變特性較好,。添加乳清分離蛋白增大了乳液的穩(wěn)定性,,降低了乳液的粘度和剪切力。

    Abstract:

    The contribution of emulsion rheological properties to soy protein isolate and whey protein isolate mixtures O/W emulsion were investigated. The whey protein isolate (WPI) was taken as emulsifier. The effects of different protein mixing ratios and concentrations on the stability and rheological properties of the composite emulsions were investigated by particle size, Zeta potential, emulsification activity index (EAI), emulsification stability index (ESI), emulsion stability coefficient, scanning electron microscopy and rheology. The results showed that when the quality score of SPI-WPI emulsion protein was 2.0% and the SPI and WPI quality ratio was 1∶9, the average volume of the emulsion was 288.56nm, the absolute value of Zeta potential was 35.0mV, and the maximum emulsification activity index (EAI) value was 108.23m2/g. The maximum emulsification stability index (ESI) was 3.78471min, and the stability coefficient was up to 93.59%. Under this condition, the emulsion stability was the best. When the concentration of SPI-WPI emulsion protein was 20% and the ratio was 9〖DK〗∶1, the viscosity of the emulsion was the largest, the shear stress of the emulsion was the largest, and the rheological properties were the best. In summary, the addition of whey protein isolate increased the stability of the emulsion and reduced the viscosity and shear force of the emulsion. The research result provided theoretical guidance for the improvement and application of emulsion stability.

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李良,張小影,朱建宇,韓璐,李楊,齊寶坤.大豆-乳清混合蛋白對(duì)O/W乳液穩(wěn)定性及流變性的影響[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2019,50(12):372-379. LI Liang, ZHANG Xiaoying, ZHU Jianyu, HAN Lu, LI Yang, QI Baokun. Effect of Soybean-Whey Mixed Protein on Stability and Rheological Properties of O/W Emulsion[J]. Transactions of the Chinese Society for Agricultural Machinery,2019,50(12):372-379.

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  • 收稿日期:2019-05-10
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  • 在線發(fā)布日期: 2019-12-10
  • 出版日期: 2019-12-10
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