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Reasonable development and utilization of brackish water, improvement of brackish water use efficiency, and reduction of secondary salinization risk under brackish water irrigation are effective measures to alleviate the shortage of fresh water resources in China. The combination of oxygenated technology and brackish water irrigation was carried out. Based on the combination of experimental research and theoretical analysis, the effects of brackish water, oxygenated fresh water, and oxygenated brackish water on the germination characteristics of wheat seeds were studied. The results showed that under the condition of brackish water with different salinities, 2g/L was more favorable for wheat seed germination. The germination number of wheat seed for four days were increased by oxygenated fresh water cultivation (dissolved oxygen concentration 9.5~22.5mg/L), but the root weight and average height of shoots were inhibited during the germination process of wheat seeds. The germination rate was decreased when the dissolved oxygen concentration exceeded 22.5mg/L, indicating that excessive dissolved oxygen concentration would inhibit the seed germination. The wheat seeds treated with different salinity brackish water under oxygenated treatment showed different appropriate dissolved oxygen concentrations. The optimum concentrations of dissolved oxygen were 19.5mg/L under 1g/L salinity, 22.5mg/L under 3g/L, and 12.5mg/L under 5g/L, respectively. In addition, based on the coupled experimental data of oxygenated brackish water treatment, an empirical model between the wheat seed germination rate, salinity, and dissolved oxygen concentration was established.