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In order to explore the effects of drip fertigation on accumulation and translocation of dry matter and nitrogen, and yield in wheat, three nitrogen (N) fertilizer levels: N1(180kg/hm 2 ), N2(240kg/hm 2 ) and N3(270kg/hm 2 ), and three water (W) levels: W1 (no irrigation at the growth stage), W2(two times irrigation at the growth stage), and W3 (three times irrigation at the growth stage), were set in two wheat growing seasons (2016—2018) by field experiment. The nine treatments were W1N1, W1N2, W1N3, W2N1, W2N2, W2N3, W3N1, W3N2, and W3N3. The results showed that dry matter accumulation of wheat plant reached its maximum at the flowering and maturing stages in two consecutive years. Compared with the W1N1 treatment, W3N2 treatment increased average dry matter accumulation at the flowering and mature stages, average dry matter translocation amount of the vegetative organs, average dry matter translocation rate, and average contribution rate of dry matter translocation to grain by 32.11%, 13.34%, 48.66%, 56.34%, 42.93%, respectively. In two consecutive years, nitrogen accumulation of wheat plant reached the maximum at the flowering and maturing stages of wheat. Compared with W1N1 treatment, average nitrogen accumulation at both flowering and maturing stages of wheat under W3N2 treatment was increased by 21.98% and 20.30%, respectively. In the mature stage of wheat, compared with W1N1 treatment, W3N2 treatment increased average nitrogen accumulation of stem and sheath, average nitrogen accumulation of cob and glume, average nitrogen accumulation of grain, average nitrogen transformation amount, average nitrogen transformation rate and average contribution rate of nitrogen transformation to grain by 20.19%, 27.65%, 35.99%, 47.51%, 20.91%, 6.04%, respectively. In two consecutive years, compared with W1N1 treatment, W3N2 and W3N3 treatment increased average wheat yield by 31.88% and 15.28%, respectively. In conclusion, the W3N2 treatment was the best in the drip fertigation, which was applied to wheat with 240kg/hm 2 pure nitrogen, 60% pure nitrogen was applied at the bottom, 25% of pure nitrogen was added at the jointing stage and 15% of pure nitrogen was added at the filling stage, respectively, and three times irrigation at the jointing stage, flowering stage and filling stage under the drip fertigation, which could promote the accumulation and translocation of dry matter and nitrogen of vegetative organs, so as to promote the high-yield and high-efficient wheat.