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A self-walking mulberry garden integrated machine with variable proportion and directional disperse fertilization was developed to balanced fertilization of nitrogen, phosphorus, potassium and organic fertilizers on demand and reduce non-point source pollution caused by unreasonable use of fertilization. The length, width and height of the integrated machine designed according to the planting row spacing of mulberry garden were 1450mm, 655mm and 1141mm, respectively, and the key components were designed. It was found that the standard deviation of the fertilizer application deviation was less than 0.4 when the roating speed of the flute-wheel was in the range of 20 ~ 80r/min. The distribution of the curved leaves was symmetrical and the fertilization effect was better. The orthogonal test was used to optimize the directional fertilization plate. The better combination of the directional fertilization plate was 450mm in length, 80mm in height and 100°in bending angle. Through response surface analysis, the influence order of the factors on the variation coefficient of fertilizer distribution was as follows: the speed of the fertilizer disperse plate, the distance between the receiving port of the collision blending device and the center of the fertilizer disperse plate, the working speed of the machine. The working parameters were optimized. The optimization results were that the speed of the fertilizer disperse plate was 290.1r/min, the distance between the receiving port of the collision blending device and the center of the fertilizer disperse plate was 88.2mm and the working speed of the machine was 0.5~0.7 m/s. It was verified that the variation coefficient of fertilizer distribution was less than 40% when the self-walking mulberry garden integrated machine working at the field experiment. The research result can provide a reference for the balanced fertilization of mulberry garden on demand.